![]() This does not require ATP hydrolysis nor is it accompanied by entrapment within S-K rings, which is a slower process requiring Scc3. ![]() Scc2 alone promotes entrapment of DNAs in the E-S and E-K compartments, between ATP-bound engaged heads and the SMC hinge and associated kleisin, respectively. Using thiol-specific crosslinking we provide rigorous proof of entrapment activity in vitro. All three activities require related hook-shaped proteins called Scc2 and Scc3. In addition to extruding DNA loops, cohesin entraps within its SMC-kleisin ring (S-K) individual DNAs during G1 and sister DNAs during S-phase. ![]()
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